The internet is filled with different methods, procedures, and workflows that can overwhelm you during data analysis, especially if this is your first time with RT-qPCR. This article is going to dive into some …

Gene expression analysis by real-time qPCR has been a key enabler of a routine and robust approach for measuring gene expression in genes of interest, as well as monitoring biomarkers. This section …

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system. For some applications, a qPCR will be …

Quantitative PCR (qPCR) is one of the foremost methods for detection and quantification of nucleic acid targets. The technique is also known as real-time qPCR and is not to be confused with RT-qPCR …

Learn statistical analysis of qPCR data for accurate fold change detection. Ensure precision in your experiments and reliable outcomes.

Dec 1, 2024 · Here, we provide an overview of RT-qPCR, focusing on essential reagents, the importance of primer design, the detailed workflow, and data analysis methods. This guide will be …

Nov 19, 2025 · Furthermore, the absence of raw data and analysis code limits the community's ability to evaluate potential biases and reproduce findings. Here, we primarily aim to encourage researchers …

By quantifying how active a particular gene is in a cell or tissue, scientists can understand how cellular activity changes in response to various conditions, such as disease or treatment. This guide covers …

ChIP analysis by qPCR works best when starting with more dilute DNA samples (as opposed to highly concentrated templates which can inhibit Taq polymerase when present in high concentrations).

Watch this short video to see one of our gene expression qPCR analysis tools in action. Or, visit the GeneGlobe Data Analysis Center to see all the analysis modules.